Isolation and Atibiotyping of Pseudomonas spp. Associated with Bovine Diarrhea

Bovine diarrhoea is a multifactorial disease involving infectious and non-infectious factors. Various pathogens of bacterial origin are responsible for the diarrhoea in cattle. Gram-negative bacteria are considered as the most common etiological agents isolated from clinical cases of diarrhea. Among Gram negative bacteria, Pseudomonas is considered as the opportunistic pathogen and associated with various problems of medical and veterinary importance including ear and urinary tract infections in dogs, mastitis and metritis in dairy cows and endometritis in horses. During the present study, 47 faecal samples were collected from the clinical cases of bovine diarrhea in Meerut city, Uttar Pradesh for isolation, identification and antibiotyping of Pseudomonas spp. Out of these faecal samples, Pseudomonas spp. could be isolated from six samples indicating the positivity of 12.77%. All the isolates of Pseudomonas spp. were subjected to in vitro antibiogram testing using 7 antimicrobials viz., enrofloxacin, gentamicin, streptomycin, amikacin, oxytetracyclin, ceftriaxone and metronidazole. All the Pseudomonas isolates were resistant to streptomycin, oxytetracyclin and metronidazole. The antibiotic susceptibility profile of the isolates was for ceftriaxone and gentamicin (50% each) and for enrofloxacin and amikacin (33.33% each). Antimicrobial sensitivity analysis of all the six Pseudomonas isolates revealed 04 different biotypes. The study revealed that isolates were found resistant to a number of antibiotics/antimicrobials ranging from 02 to 07. The isolates showing more susceptibility to enrofloxacin and amikacin may be used for the treatment of bovine diarrhea associated with Pseudomonas spp. This further recommended the use of routine antibiotic sensitivity testing before the treatment to avoid dissemination of antimicrobial resistance among microbes.