1PG Scholar, Department of Veterinary Microbiology, Bihar Veterinary College, BASU, Patna, Bihar, India
2Assistant Professor, Department of Veterinary Microbiology, Bihar Veterinary College, BASU, Patna, Bihar, India
3Assistant Professor, Department of Veterinary Public Health, Bihar Veterinary College, BASU, Patna, Bihar, India
4Associate Professor, Department of Veterinary Microbiology, Bihar Veterinary College, BASU, Patna, Bihar, India
5Associate Professor, Department of Veterinary Public Health, Bihar Veterinary College, BASU, Patna, Bihar, India
6Ph.D. Scholar, Department of Veterinary Public Health, Bihar Veterinary College, BASU, Patna, Bihar, India
*Corresponding author email id: drsavitakumari@gmail.com
Online Published on 26 May, 2023.
The ability of uropathogenic Escherichia coli to grow and initiate infection in the uroepithelium is associated with the ability to form biofilms and the presence of different virulence factors. Biofilm production was detected in 21 urinary E. coli isolates obtained from bovine by Congo red agar morphotyping. Red, dry and rough (RDAR) morphotype was shown by 10 isolates, whereas 04 isolates produced the Brown, dry and rough (BDAR) morphotype and 07 isolates gave the smooth and white (SAW) morphotype. In this present study, 66.67% (14/21) of E. coli RDAR and BDAR morphotypes producing isolates were found associated to form biofilm, whereas, PCR detection revealed the presence of fimH gene in 71.43% (15/21) isolates by producing specific 164 bp amplicon. The knowledge of the virulence genes and biofilm formation may be helpful for the timely and appropriate treatment and the management of urinary tract infection.
Escherichia coli, Biofilm, Congo Red Agar, Fimh gene
