Journal of Immunology and Immunopathology
  • Year: 2022
  • Volume: 24
  • Issue: 2

Occurrence and serotyping of Escherichia coli isolates from milk and milk products

  • Author:
  • Pragya Nema1, R.V. Singh2,*, Bhavana Gupta2, R. Kulesh2, Brejesh Singh3, Kajal Kumar Jadav4, Girraj Goyal5
  • Total Page Count: 5
  • Published Online: May 26, 2022
  • Page Number: 118 to 122

1MVSc Scholar, Department of Veterinary Public Health & Epidemiology, College of Veterinary Science & Animal Husbandry, NDVSU, Jabalpur-482001, Madhya Pradesh, India

2Professor & Head, Assistant Professor, Department of Veterinary Public Health & Epidemiology, College of Veterinary Science & Animal Husbandry, NDVSU, Jabalpur-482001, Madhya Pradesh, India

3Assistant Professor, Department of Veterinary Public Health & Epidemiology, College of Veterinary Science & Animal Husbandry, NDVSU, Jabalpur-482001, Madhya Pradesh, India

4Assistant Professor, Centre of Wildlife Forensic & Health, NDVSU, Jabalpur-482001, Madhya Pradesh, India

5Assistant Professor, Department of Poultry Science, College of Veterinary Science & Animal Husbandry, NDVSU, Jabalpur-482001, Madhya Pradesh, India

*Corresponding author email id: rvsvet24oct@gmail.com

Online Published on 26 May, 2023.

Abstract

The present study was conducted to observe the occurrence of Escherichia coli in milk and milk products. A total of 244 samples of milk and milk products were examined by enrichment in MacConkey lactose broth followed by plating on MacConkey Lactose Agar. An overall occurrence of 29.09% E. coli was observed. The highest occurrence was found in raw milk (53.01%) followed by pasteurized milk (21.41%), ice-cream (20.75%) and shrikhand (7.69%). In raw milk, the highest occurrence was observed in milk collected from vendors (63.3%) followed by dairies (53.3%) and homes (39.1%). Similarly, the study in pasteurized milk revealed 25.8%, 21.0%, and 0.0% presence of E. coli in brand-I, brand-II and brand-III, respectively. In ice-cream, the local samples showed the highest presence of E. coli (27.7%) followed by brand-II (21.4%) and brand-I (14.2%). The presence of E. coli in local shrikhand (20.0%) was higher than branded items of shrikhand. The χ2 value 32.51** indicates that there was a highly significant difference among the samples for E. coli contamination.

Keywords

E. coli, Milk and milk products, Occurrence, Serotyping, Isolates