Oxidative Damage, Haemato-Biochemical Alterations in Broiler Chicks Exposed to Chlorpyrifos and Antioxidative Role of Selenium

The present study was undertaken to evaluate the antioxidative role of selenium against chlorpyrifos-induced oxidative and haemato-biochemical changes in broiler chicks. A total of fifty-four chicks were randomly divided into three equal groups. The chlorpyrifos was given @ 3 mg/kg body weight daily in birds of Group II and Group III. The feed of the birds of Group III was also supplemented with sodium selenite @ 0.25 ppm. The birds of the Group I were kept as control. Six broilers from each group were slaughtered at Day 14-, 28- and 42 post feeding for the study of oxidative damage and haemato-biochemical changes. The oxidative enzymes, Lipid peroxidation (LPO) were significantly increased while the reduced glutathione (GSH) decreased significantly in the birds of Group II and Group III as compared to birds of Group I. In contrast, co-administration of selenium to CPF-treated birds restored most of these oxidative parameters were less severe and low frequency at different time intervals in birds of Group III. Haematological observations revealed significant decrease in the values of Hb, PCV and TEC. The values of TLC revealed significant decrease at all intervals with heterophilia and monocytosis with significant lymphocytopenia in Groups II. The values of AST and ALT were significantly increased in between the groups and in between the intervals. The ALP revealed significant increase in the birds of Group II on Day 42 as compared to control. There was significant increase in the level of glucose, urea and creatinine and decrease in total protein level at different time intervals in Group II. However, selenium administration to CPF-treated birds resulted in overall improvement in haemato-biochemical alterations. Observations from the present study that selenium supplementation can be ameliorate chlorpyrifos induced toxic effects in broiler chicks.