Journal of Immunology and Immunopathology

In October 2023, an African Swine Fever (ASF) outbreak occurred on a pig farm in Sivsagar district, Assam, resulting in infection in all the pigs and death of three pigs. Tissue samples were obtained from these deceased pigs for further investigation. A total of 18 tissue samples were screened using Polymerase Chain Reaction (PCR) to detect the presence of viral DNA. Strikingly, 7samples were confirmed positive for both African Swine Fever Virus (ASFV) and Porcine Circovirus Type 2 (PCV2). This robust confirmation through PCR highlights the coinfection of ASFV and PCV2 in the studied pig population. Concurrently, a comprehensive post-mortem examination was conducted, revealing gross pathological changes, and tissue samples were collected for histopathological analysis. The subsequent histopathological examination unveiled distinct lesions consistent with ASF, further corroborating the presence of ASFV. These findings not only underscore the prevalence of ASFV and PCV2 coinfection but also provide essential insights into the histopathological consequences of the viral infections in the examined pig population. This dual approach of PCR and histopathology enhances the comprehensiveness of the study, contributing significantly to our understanding of the viral dynamics and aiding in the formulation of targeted interventions for disease management in swine populations.