Journal of Immunology and Immunopathology
  • Year: 2001
  • Volume: 3
  • Issue: 1

Detection of Salmonella gallinarum antibodies in chickens by Dot-enzyme linked immunosorbent assay

  • Author:
  • D. Madhuri2, Puran Chand1, J.R. Sadana1
  • Total Page Count: 4
  • Page Number: 46 to 49

1Department of Veterinary Pathology, CCS Haryana Agricultural University, Hisar, Haryana - 125 004, INDIA.

2AICRP on Poultry for Eggs, Livestock Research Unit, Rajendra Nagar, Distt. Rangareddy, Andhra Pradesh-500 030.

Abstract

Antibodies to Salmonella gallinarum were detected by a Dot-enzyme linked immunosorbent assay (Dot-ELISA) in broiler chickens experimentally infected with S. gallinarum var duisburg. All the infected chickens were found serologically positive when examined at one week post-infection. Very high antibody titres were recorded in the first week which continued up to 4th week of infection and then started declining. The Dot-ELISA was found to be a sensitive, rapid and simple assay for detecting S. gallinarium antibodies in chickens.

Keywords

Poultry, Dot-ELISA, Salmonella gallinarum