1Department of Veterinary Clinical Medicine, Ethics and Jurisprudence, COVS, CCS Haryana Agricultural University, Hisar-125004, INDIA.
2National Research Centre on Equines, Sirsa Road, Hisar-125001, INDIA.
Otitis externa and seborrheic dermatitis in dogs are frequently associated with Malassezia pachydermatis. which is a unipolar budding, lipophilic normal commensal yeast. Protein fraction of two M. pachydermatis isolated from dogs suffering from otitis externa were determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). The electrophoretic runs of both the antigens resulted in 21 discrete fractions ranging in molecular weights from 19.10 kDa to 123.02 kDa. The molecular weight of these fractions were 19.10, 20.09, 20.32, 21.67, 22.59, 23.93, 26.66, 28.11, 30.13, 30.76, 33.97, 36.22, 38.81, 44.05, 47.90, 50.93, 60.11, 67.61, 77.09, 89.54, and 123.02 kDa. This is the first report on protein profiling of M. pachydermatis in dogs in India.
Malassezia pachydermatis, Crude extract, SDS PAGE, Otitis externa, Dog
