1AICRP on Blood Protista, College of Veterinary Sciences, CCS HAU, Hisar-125 004.
2Department of Biotechnology, Guru Jambeshwar University, Hisar-125 001, (Haryana).
An indirect immunoperoxidase test (IPT) was standardised using in vitro propagated Theileria annualta (Hisar) schizonts as antigen. The specificity and sensitivity of IPT was compared with conventional indirect fluorescent antibody test (IFAT) for sero-epidemiological studies on T. annulata. IPT was found to be more sensitive, specific and easier to interpret than IFAT. The slides stained with IPT could be stored for longer periods than IFAT. A good correlation was observed between antibody titres obtained using these two tests. However, antibody titres obtained by IPT were significantly higher than those obtained by IFAT. Seroprevalence studies in three districts of Haryana using IPT revealed that 72.64% cattle were positive for presence of antibodies against T. annulata.. IPT is recommended in place of conventional IFAT for serological screening of field animals for tropical theileriosis.
Indirect immunoperoxidase test, Indirect fluorescent antibody test, Theileria annulata., Schizonts
