College Central Laboratory, College of Veterinary Sciences, CCS Haryana Agricultural University, Hisar-125 004, (Haryana), INDIA. E-mail: vetyccl@hau.nic.
Anti-bovine IgG peroxidase conjugate was used for detecting Brucella antibodies in sera of cattle and buffaloes by Enzyme Linked Immunosorbent Assay (ELISA). The serum samples from cattle and buffaloes of known status for brucellosis were analysed. The anti-bovine IgG peroxidase conjugate produced expected results with cattle as all the Brucella positive cattle sera exhibiting STAT titres between 1:20 and 1:640 with positive RBPT reaction gave OD values above 1.000 (range 1.061 to 1.996). While in buffaloes that exhibiting similar STAT titres (i.e. 1:20 to 1:640) with positive RBPT reaction the OD values in ELISA remained below 0.700 (range 0.183 to 0.682). Though anti-bovine IgG peroxidase conjugate showea cross-reactivity with buffalo sera in ELISA but the strength of signal in terms of OD values was much lower than the cattle sera. Use of anti-bovine IgG peroxidase conjugate in ELISA for detecting Brucella antibodies in buffaloes could lead to erroneous results.
Anti-bovine IgG peroxidase conjugate, ELISA, Brucella antibodies, Cattle, Buffaloes
