comparative study on use of sodium perborate in place of hydrogen peroxide in ELISA

Different enzyme labels are used with different chromogenic substrate mixtures for developing ELISA. Horse-radish peroxidase enzyme alongwith O-phenylenediamine (OPD) and hydrogen peroxide (H₂O₂) is one of the commonly used systems. Use of solution of H₂O₂ poses many problems including loss in activity with time. In this study, sodium perborate was evaluated for its efficiency as substitute for H₂O₂. Anti Pasteurella multocida outer membrane protein antibody titres for 92 cattle sera samples were calculated by end pomt indirect ELISA using the substrate solution with sodium perborate or solution of H₂O₂. The antibody titres obtained by using these two substrate systems were found comparable. Therefore, sodium perborate could be used in the chromogen-substrate buffer in place of solution of H₂O₂ without having any ill or negative effect on performance of ELISA.