1Part of M.V.Sc. Thesis of first author approved by Acharya N.G. Ranga Agricultural University, Tirupati.
2Department of Veterinary Microbiology, C.V.A.Sc., G.B. Pant University of Agriculture and Technology, Pantnagar, (Uttaranchal).
3Department of Microbiology, C.V.Sc., Acharya N.G. Ranga Agricultural University, Tirupati-517 502, (AP), India.
comparison of c-ELISA with i-ELISA was done with 390 field sera of different species to evaluate their relative efficacy in monitoring antibody response to HS vaccination. Capsular and OMP antigen were employed for plate coating in both the ELISA system. The results of both the assays were compared to find a sensitive, specific and convenient test for routine assessment of immune status in vaccinates. The mean antibody titres detected by both capsular and OMP antigens do not show any significant difference. The performance of c-ELISA relative to i-ELISA was found to have a sensitivity of 92.4% and specificity of 81.6% with overall agreement of 88.97% using capsular antigen. Similarly, the relative sensitivity, specificity and overall agreement between c-ELISA and i-ELISA with OMP antigen were 86.8%, 84.61% and 86.1%, respectively. Both the tests are more or less equally sensitive. Further, the c-ELISA is found to be more useful tool for routine seromonitoring as it eliminates the need of different conjugates for each species.
Haemorrhagic septicaemia, P. multocida P52, Seromonitoring, ELISA
