1Dept. of Veterinary Epidemiology & Preventive Medicine, College of Veterinary Sciences, CCS Haryana Agricultural University, Hisar-125 004, (Haryana), INDIA.
2Dept. of Veterinary Public Health & Epidemiology, College of Veterinary Sciences, CCS Haryana Agricultural University, Hisar-125 004, (Haryana), INDIA.
3Government Veterinary hospital, Uttaradabas, Hanumangarh, (Rajasthan), INDIA.
4Dept. of Veterinary Microbiology, College of Veterinary Sciences, CCS Haryana Agricultural University, Hisar-125 004, (Haryana), INDIA.
*E-mail: mahajannk@hau.nic.in
**E-mail: gitamalik2@rediffmail.com
***E-mail: jaypeedahiya@rediffmail.com
Cell surface proteins of Brucella melitensis Rev-1 (BCSPs) were analysed for their protective efficacy in a passive transfer assay. Spleens of BCSPs immunized donor BALB/c mice were removed aspetically and the T-cell suspensions were made. The antisera, splenic T-cells (3×107 viable cells) and mixed spleen cells (T+B lymphocytes as 3×107 viable cells) from donor BALB/c mice were injected intravenously in recipient BALB/c mice. After 2 hrs of this injection the mice of recipient groups were challanged with 5×104 Colony forming units (Cfu) of B. melitensis S16M. The highest (1.96 log units) protection against challenge was induced by transfer of serum followed by mixed spleen cells and T-cells transfer with 1.46 and 1.07 log units, respectively.
Brucella melitensis Rev-1, Brucella Cell surface proteins (BCSPs), Immunoprotective efficacy, Passive transfer assay
