1Department of Veterinary Anatomy and Histology, Orissa University of Agriculture and Technology, Bhubaneswar-751 003, (Orissa), INDIA. E-mail: uk_ mishra2001@yahoo.com.
2Department of Veterinary Anatomy, GB Pant University of Agriculture and Technology, Pantnagar, 263145, (Uttaranchal), INDIA. E-mail: singhgk2003@yahoo.com.
3Department of Veterinary Pathology, GB Pant University of Agriculture and Technology, Pantnagar, 263145, (Uttaranchal), INDIA. E-mail: rs_chauhan123@rediffmail.com.
T-Iymphocytes are the key associates of cell mediated immunity (CMI). Development of CMI during post hatch periods in chicken was assessed using lymphocyte stimulation test (LST) with conA as the mitogen and delayed type hypersensitivity reaction (DTH) with dinitrochlorobenzene as allergen. The mean delta 00 of conA stimulated T-cell cultures was 0.033, 0.119, 0.241, 0.26, 0.327, 0.338 and 0.295 on 1, 10, 20, 30, 40, 50 and 60 days of post hatch development, respectively. The gross lesions, after challenge by the allergen, included erythema, induration, ulceration and scab formation. The mean skin thickness at 0, 12, 24, 36, 48 and 60 h post challenge recorded 2.20 ± 0.15 mm, 3.51 ± 0.11 mm, 3.76 ± 0.13 mm, 3.80 ± 0.19 mm, 3.70 ± 0.11 mm, and 3.20 ± 0.10 mm, respectively. These values for control skin were 2.10 ± 0.01 mm, 2.10 ± 0.11 mm, 2.50 ± 0.13 mm, 2.60 ± 0.19 mm, 2.50 ± 0.17 mm and 2.50 ± 0.12 mm on respective hours of observation.
Chick, cell mediated immunity, delayed type hypersensitivity reaction, aging, development, T-Iymphocytes
