National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, (Uttar Pradesh), INDIA. E-mail: sbarua@scientist.com, raia1@rediffmail.com.
The restriction enzyme analysis of fowl adenovirus 4 DNA was carried out with the enzymes Xbal, BamHI, Dral, EcoRI, Hindlll, Bgl ll, Pstl, Smal, Notl, Ndel and Sacl in order to ascertain the restriction profile. The restriction profile of FAV4 DNA (Indian isolate) was similar to the restriction profile of FAV4 reference strain KR-5. The total genomic size of FAV4 DNA was estimated to be 46 kb. Comparison of restriction profile of FAV4 (Indian isolate) with that of FAV10 revealed considerable variation with the enzymes BamHI, Bglll, Dral and Smal. The study revealed that restriction enzyme analysis could be a good basis for differentiation of FAV strains.
Virus, fowl adenovirus4, DNA, restriction enzyme
