In vitro exposure of captafol decreases production of IL-1 and IL-2 by avian mononuclear cells

Immunopathology is an emerging field in veterinary and medical that lacks versatile standardized in vitro test models. In recent decade there is a great emphasis on evaluation of immunosuppression due to environmental pollutants especially pesticides. In vitro effects of fungicide captafol on the production of IL-1 and IL-2 was studied in avian splenic mononuclear cells. The cells were treated with NOEL (No observable effect level)/10² to NOEL/10⁷ dilutions of captafol for 0.5, 1.0 and 1.5 hr and the cells were cultured in RPMI-1640 media with 5% fetal calf serum for 72 hrs at 37°C in CO₂ incubator. Cell culture fluid was collected for determinate of IL-1 and IL-2 using monoclonal markers through ELISA. The mean ELISA values of captafol treated cells showed the decreased production of IL-1 and IL-2 in comparison to controls. It was concluded that in vitro exposure of captafol decreases the IL-1 and IL-2 production.