Anti-CD3 induces T-cell signalling towards Th-1 type response in BALB/c mice immunized with 57 kDa major antigenic Omps of Shigella dysenterie type 1

Antigen alone may not be able to trace the specific path through which it can generate antigen-specific responses against shigellosis though it requires a co-stimulatory molecule. The present study was carried out to identify the immunoregulatory role of T-cell induced by anti-CD3 in Balb/c mice immunized with 57kDa antigenic component of S. dysenterie 1 in order to generate antigen-specific agnostic T-cell responses. In immunoblot analysis, we have noted that p70 of IP3 mediating receptors activated during T-cell signalling and phosphorylated by the PTKase through intercellular calcium ([Ca²⁺]i) might help in generating IL-2 rather than IL-4. The present data suggest that agnostic signals for CD4+ T-cells are up regulated by IP3-mediated signals in anti-CD3 stimulated cells of immunized mice.