Detection of virulence in Indian isolates of Salmonella by polymerase chain reaction
Abstract
Virulence in Salmonella is reported to be caused by the large plasmids of 60‒100 Kb. In the present study, 24 strains of four serovars (S. Dublin, S. Abortusequi, S. Bareilly and S. choleraesuis) were studied for their in vivo virulence, plasmid profiling and virulence (Vir) gene detection by Polymerase Chain Reaction. Out of 24 strains, 19 strains were found to be virulent inducing different degrees of mortality. In 5 non-virulent strains large plasmid was found to be absent and there was no amplification of virulence gene by PCR. This clearly indicate a positive correlation between virulence of organism and the presence of plasmid and amplification of Vir gene.
