1Department of Microbiology and Molecular Biology, Central JALMA Institute for Leprosy and Other Mycobacterial Diseases (ICMR), Taj Ganj, Agra.
2S.N. Medical college, Agra.
3 Department of TB and Chest Diseases, S. N. Medical College, Agra.
The rpoB gene could be amplified from DNA extracted from all the 55 culture isolates and it showed clear hybridization pattern with M. tuberculosis complex specific probes on LiPA strips. Mutations detected were correlated with degree of rifampicin resistance. All the sensitive strains (identified by MIC) were identified as rifampicin sensitive (100%) by INNO-LiPA. This method could diagnose with definitiveness 60%(21/35) isolates as rifampicin resistant as mutations observed in others were also present in isolate with low levels of resistance. These results indicate that INNO-LiPA Rif TB test is a rapid and easy to use method for detection of mutations associated with rifampicin resistance in M. tuberculosis. These results indicate that INNO-LiPA Rif TB test is a rapid and easy to use method for detection of mutations associated with rifampicin resistance in M. tuberculosis.
M. tuberculosis, Sensitivity, Mutation detection, rpoB gene analysis
