Journal of Immunology and Immunopathology

UGC CARE (Group 1)
  • Year: 2005
  • Volume: 7
  • Issue: 2

Double antibody sandwich ELISA for detection of staphylococcal antigens in milk from sub-clinical mastitis cases of cows

  • Author:
  • Arun Kumar Jain1, Parveen Goel1, Anshu Sharma2, Ravindra Sharma3
  • Total Page Count: 4
  • DOI:
  • Page Number: 34 to 37

1Department of Veterinary Clinical Medicine, Ethics and Jurisprudence, CCS Haryana Agricultural University, Hisar, 125 004, (Haryana), India

2Veterinary College Central Laboratory, Regional Research Centre on Foot and Mouth Disease, CCS Haryana Agricultural University, Hisar, 125 004, (Haryana), India

3College of Veterinary Science, CCS Haryana Agricultural University, Hisar, 125 004, (Haryana), India

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Abstract

Double antibody sandwich ELISA (DAS-ELISA) was developed for detection of staphylococcal antigens in milk samples from sub-clinical mastitis cases of cows. Out of 279 quarter milk samples from 72 apparently healthy lactating crossbred cows screened for staphylococcal mastitis, 41.22% and 12.90% milk samples were detected for Staphylococcus. aureus and S. epidermidis antigens by DAS-ELISA specific for S. aureus and S. epidermidis, respectively. In cultural examination, 42.29% were found positive for sub-clinical mastitis. DAS-ELISA was found rapid, cheaper, sensitive, specific and less cumbersome as compared to cultural examination. DAS-ELISA system specific for S. aureus was found to be used for screening the herd for both S. aureus and S. epidermidis mastitis whereas DAS-ELISA specific for S. epidermidis was useful assay for detection of S. epidermidis mastitis only.

Keywords

Bovine sub-clinical mastitis, Double antibody sandwich ELISA, Staphylococcus aureus, Staphylococcus epidermidis