1Department of Pathology, G B Pant University of Agriculture and Technology, Pantnagar, 263 145 Udham Singh Nagar (Uttaranchal)
2Department of Anatomy College of Veterinary Sciences, G B Pant University of Agriculture and Technology, Pantnagar, 263 145 Udham Singh Nagar (Uttaranchal)
3CADRAD, IVRI, Izatnagar, Bareilly, (Uttar Pradesh) INDIA
*Corresponding author: E-mail: seema_patho@rediffmail.com
The effect of gamma-BHC on humoral immune response in chickens was studied. Chicks were vaccinated with Ranikhet disease vaccine (RDV) at day 4 and Infectious Bursal Disease (IBD) at day 15. Group-I chicks were kept as control while group-II was given gamma-BHC daily at a dose rate of 25 ppm orally for 2 months in feed. Serum globulin and gamma globulin were estimated in serum at one week interval. Specific humoral immune response against RDV and IBD vaccine was measured by ELISA. B-lymphocyte blastogenesis assay was performed using lipopolysaccharide (LPS) as mitogen. There was a significant suppression in serum globulin, gamma-globulin and specific antibody titre against RDV and IBDV. B-lymphocyte in blastogenesis was found to be reduced in chicks fed gamma-BHC in comparison to control indicating immunosuppressive effect of gamma-BHC.
Gamma-BHC, IBD vaccine, immunopathology, Ranikhet disease vaccine
