Department of Veterinary Microbiology, CCS Haryana Agricultural University, Hisar-125004 Haryana.
The double antibody sandwich ELISA based on baculovirus expressed non structural protein (NSP) 3A of FMD virus and monoclonal antibody against NSP 3A, was used for differentiation of Foot-and-Mouth Disease (FMD) infected and vaccinated animals (DIVA) using milk samples from buffaloes from rural cohort. The antibodies against 3A NSP were detected in 8.8% of milk samples.
Foot-and-mouth-disease, carriers, NSP, DIVA, NSP-ELISA, Milk antibodies