Seromonitoring of cattle and buffaloes vaccinated against Haemorrhagic septicaemia using monoclonal antibody based indirect-ELISA

Seromonitoring of 156 buffaloes and 64 cattle sera samples for Haemorrhagic septicaemia (HS) was carried out by using monoclonal antibody based indirect ELISA (Mab-ELISA). The mean ELISA titers (log₁₀) in buffaloes against aluminium hydroxide gel precipitated H.S. vaccine, four months post vaccination in Gurgaon, Karnal, Jind and Sirsa districts were found to be 2.22±0.06, 2.09±0.02, 3.46±0.08 and 2.14±0.03, respectively, and were found to be protective, whereas at six months post vaccination they were marginally protective. The mean antibody titers (log₁₀) in cow heifers, Hariana cattle and Jersey crossbred were found to be 1.51± 0.02, 1.78±0.05 and 1.67±0.15 respectively, three months after first vaccination with HS and Black quarter (BQ) combined vaccine The titers increased significantly (P<0.1) to 2.09±0.07, 2.64±0.13 and 2.36±0.15 in these three groups of cattle respectively, three months after second vaccination. Both aluminium hydroxide precipitated HS vaccine as well as aluminium hydroxide gel adsorbed HS and BQ combined vaccine elicited protective immune response in animals