Assessment of antihyperglycemic and antihyperlipidemic activity of Spondias mangifera willd root

The present study was designed to investigate the antihyperglycemic and antihyperlipidemic activity of methanol extract of roots of Spondias mangifera Willd in rats. The test was performed in both normoglycemic and alloxan induced hyperglycemic rats under treatment of the tested methanol extract at 200 and 400 mg/kg dose levels for 30 days, and glibenclamide (2.5 mg/kg) was used as standard drug for activity comparison. The biochemical parameters used in the study are blood glucose, plasma-insulin and lipid profiles determination was performed using standard experimental procedure. The test result revealed that in normoglycemic rats the blood glucose level reduces to a significant (p<0.05) extent in a dose-dependent manner, while the hyperglycemic rats showed a progressive fall of blood sugar level in a significant extent (p<0.05 to 0.001). The extract at the tested dose levels, significantly (p<0.05) increases the peripheral utilization of glucose by isolated rat hemi-diaphragm.

It was concluded that, the remarkable antidiabetogenic effect exerted by the methanol root extract of Spondias mangifera. The % entrapment efficiency and % drug loading of GLPC (86.02±0.18%, 08.26±0.72%) and GP (84.36±0.42%, 08.05±0.03%) was found, respectively. The in vitro release rate of GP (86.03±0.06%) was slower than GLPC (88.93±0.33%) in pH 7.4 phosphate buffer up to 24 h by diffusion process (Korsmeyer Peppas model). GLPC has shown the potent antioxidant activity, susceptible antibacterial activity and significant anti-inflammatory activity as compared to GP. GLPC has improved the significant bioavailability and also correlate the hematological values on rabbit blood against the incubation of microorganisms (S. aureus & E. coli). The prepared nanoparticle based complex of phytosome loaded of phyto-constituent drug has the combined effect of chitosan and phytosome which shown better sustained-release profile and also prolonging the oral absorption rate of gingerol with effective antibacterial activity in a better stable way at different storage conditions than phytosome or drug with chitosan.