Synthesis Characterization and Biological Evaluation of Some Novel Substitute D-1, 3-Thaizine Congeners

In the present investigation, a new series of 2-amino-4-phenyl-6H-1, 3-thiazine indolin-2-one derivatives (3a-j) were synthesized from 1, 3-thiazine derivatives. In the beginning acetophenone which underwent Claisen-Schmidt condensation reaction, was dissolved in ethanol and various substituted aromatic aldehydes were added in the presence basic media to afford , β unsaturated compounds (1a-j). Further these various chalcones were subjected to cyclocondensation with thiourea, in ethyl alcohol, catalyzed by aqueous potassium hydroxide to afford 1, 3-thiazine derivatives (2a-j). Now these 1, 3-thiazine derivatives were refluxed with substituted isatin catalyzed by glacial acetic acid in ethanol to afford 2-amino-4-phenyl-6H-1, 3-thiazine indolin-2-one derivatives (3a-j). The structures of the newly synthesized compounds have been characterized by their IR, 1H NMR, 13C NMR, MS spectral data and elemental analysis

These newly synthesized compounds were screened for their In vitro and in-vivo anti-inflammatory activity at the dose of 100μg, and 200 mg/kg per oral. In vitro anti-inflammatory activity was evaluated using albumin denaturation assay and in-vivo anti-inflammatory activity by Carrageenan induced rat paw oedema model. Diclofenac sodium was used as standard drug. The biological result shows that some of the compounds were good in their anti-inflammatory activity.