Division of Sail Science and Agricultural Chemistry, Indian Agricultural Research Institute, New Delhi, 110012
In adsorption study of C1—cellulose and Cx—cellulose derived from Trichoderma reesei (isolatedfrom decomposing wheat straw), on kaolinite and bentonite saturated with sodium, calcium and aluminium, an equilibrium was attained within 60 min. for both the cellulose components. Adsorption of celluloses increased linearly up to a clay concentration of 100 mg/ml, bentonite adsorbing more than kaolinite. Adsorption on different homoionic clays decreased in the following sequence: Al clay> Ca clay>Na clay. The amount of enzyme adsorbed increased with equilibrium concentration and in general, L type adsorption isotherms were obtained. Sorption maxima (Xmax) values were higher for C1—cellulose than for Cx—cellulose suggesting preferential adsorption of the former. The maximum adsorption of both the celluloses on the two clays was around pH 5.0 which is also optimum for cellulose hydrolysis
Cellulose hydrolysis, cellulase soil enzymes, adsorption, clay minerals, clay-enzyme interaction
