1PhD Student, Department of Forensic Medicine & Toxicology, All India Institute of Medical Sciences, New Delhi, 110029
3Professor, Department of Forensic Medicine & Toxicology, All India Institute of Medical Sciences, New Delhi, 110029
#Corresponding author: Professor, Department of Forensic Medicine & Toxicology, ins , New Delhi, 110029; Email: dradarshk@yahoo.com, Tel-09868438856
Online published on 21 August, 2018.
Suicidal and accidental poisoning with drug overdose is one of the common causes of morbidity and mortality in India. Considering such fact, quantification of drug becomes imperative to distinguish narrow margin of therapeutic dose and toxic dose. Acetaminophen (Paracetamol) is extensively used as antipyretic and analgesic drug. Generally it is used in headaches, slight pains and fever. Overdose of Paracetamol causes hepatotoxicity. It can be fatal in large dose (>10g). It can potentiate the harmful effect of other drugs if taken in combination. A quick, simple and precise method is developed for quantitative estimation of acetaminophen using RP-HPLC in biological samples viz. Blood, Vitreous Humor and CSF. The separation was done using RPC-18 HPLC column and Methanol: water as mobile phase. On the basis of ICH guidelines validation parameters such as Linearity, Accuracy, Precision, Recovery, Limit of Quantification and Limit of Detection were done.
Acetaminophen Paracetamol Validation RP-HPLC Quantification
