The development of the spraying reagent for the detection of the bromadiolone in thin layer chromatography.

Over the years, Forensic Science Laboratories receives cases of accidential as well as intentionall bromadiolone intoxicaton. Bromadiolone is an Anticoagulant inhalant rodenticide used in rural and urban areas to control rodent. Over the last many decades, it has been observed that the use of rodenticide has been increased in promotng public health in hospitals, outdoor and home, with many generations coming into existence. The easy availability of rodenticide for use in public hygiene and agricultural has caused them to be used for suicidal and homicidal purposes. Bromadiolone, one of the most commonly available rat kill poison acts by inhibiting the synthesis of Vitamin K in blood, thereby causing internal bleeding and hemorrhage. Reported literature on the detection of bromadiolone poisoning mention detection of TLC spot by visualizaton under UV light only. The present study aimed for developing spray reagent for visualizaton of the Bromadiolone spots on Thin layer chromatography plates.