1Department of BCI, College of Horticulture, University of Horticultural Sciences, Bagalkot, 587104, (Karnataka), India
2Department of NRM, College of Horticulture, University of Horticultural Sciences, Bagalkot, 587104, (Karnataka), India
3Department of PSMA, College of Horticulture, University of Horticultural Sciences, Bagalkot, 587104, (Karnataka), India
*Corresponding Author’s Email - jayaram.naik@uhsbagalkot.edu.in
Online Published on 30 July, 2025.
A simple assay for the estimation of ethanol concentration is essential during the extraction of bioactive compounds in botanical research. This study presents an assay developed to measure ethanol levels in different extracted samples. Utilizing the boiling point properties of ethanol in conjunction with a hot air oven, the method is capable of determining a minimum standard alcohol content of 2% by volume, with a precision of ± 0.5%. This technique proves particularly advantageous for identifying ethanol that may have been inadvertently collected alongside the target compounds during the extraction process. Furthermore, this assay has been demonstrated to be a cost-effective, simple and accurate means of determining ethanol concentration in unknown solutions.
Bioactive Compounds, Boiling Point, Ethanol, Plant Extract, Simple Method
