in vitro protocol standardization for growth and rooting in strawberry

Strawberry is commercially propagated via runner multiplication but this method being laborious and hectic could not meet the demand of local fresh market, processing and export. Also, the health and quality of plant is degenerated and not up to mark by this method. Hence, the present study was conducted at Guru Kashi University, Talwandi Sabo, Bathinda during 2017-2018 with an aim to study in vitro explant response and regeneration capacity of strawberry cultivar sweet Charlie using different media composition through micro propagation. For explant, meristem gave superior and significantly better results than nodals. Also, best effect came due to the treatment DEM₁ with respect to regeneration percentage, shoot length and leaves. The interaction between the two factors concluded that it was better to use meristem as explants and DEM₁ as media composition for better regeneration potential and vegetative growth. For rooting, REM₁ media composition showed best rooting response with minimum days taken (6) and highest rooting percentage (80%).