Biotechnology Laboratory, Division of Horticulture, ICAR Research Complex for NEH Region; Umiam, 793 103, Meghalaya. e-mail:flori_rajiv@yahoo.co.in
In vitro propagation of Thunia marshalliana Rchb f., an epiphytic-cum-terrestrial orchid is not available at present; thus efforts were made to develop an effective protocol for in vitro propagation and plantlet development using embryo as an explant source. The influence of medium, Activated charcoal (AC) on embryos (seeds), protocorm like bodies (PLB) formation, differentiation, plantlet development and growth of Thunia marshalliana was assessed. Results revealed that maximum germination (79.33 and 72.68%) was occurred on Phytamax and MS medium, respectively. The morphogenetic responses of PLBs were further assessed on Phytamax and MS medium and their combination with different levels of Activated Charcoal. The media supplemented with AC had a marked effect on plantlet growth and AC 3g/l was found to be the best for plantlet growth.
Activated Charcoal, In vitro propagation, MS medium, Thunia marshalliana
