1Department of Biotechnology,
2Department of Floriculture and Landscaping, College of Horticulture, Dr. Yaswant Singh Parmar University of Horticulture and Forestry, Nauni, Solan-l73230, Himachal Pradesh e-mail: shirkotcp@yahoo.co.in
The isozyme analysis was conducted for nine enzyme systems and seven of them showed significant activities providing typical banding patterns. Four enzyme systems, NADH dehydrogenase, peroxidase, a-esterase and aspartate amino transferase showed considerable and veryuseful variation among 13 carnation (Dianthus caryophyllus Linn.) cultivars. Allele frequency in the present set of cultivars ranged from 0.5 to 1.0 and at polymorphic loci only two alleles could be identified. Polymorphism in the selected 13 cultivars was found to be 13.8 per cent. A dendrogram showed grouping of cultivarsin two clusters on the basis of genetic distance values and there were three sub-clusters.
Carnation, genetic marker, isozymes, polyacrylamide gel electrophoresis
