Department of Floriculture and Landscaping, Horticultural College and Research Institute, Tamil Nadu Agriculture University, Coimbatore -641 003, Tamil Nadu.
A protocol was developed for in vitro propagation of Petunia hybrida Vilm. Multiple shoot formation was noticed on Murashige and Skoog's medium supplemented with 3 mg 1−1 6 benzylaminopurine from stem explants and 2 mg 1−1 6−benzylaminopurine from axillary bud explants. Shoot elongation occurred on MS medium supplemented with 0.5 mg 1−1 and 0.75 mg 1−1 of gibberellic acid for stem and axillary bud explants. Efficient rooting was achieved on half strength MS medium supplemented with 0.5 mg 1−1 indole 3-butyric acid and 0.5 mg 1−1 indole 3-acetic acid. The regenerated plantlets were acclimatized and successfully transferred to the ex vitro condition.
Stem explants, multiple shoot formation, axillary bud explants, shoot elongation, root formation
