Improved micropropagation protocol in Lilium hybrida var. Pollyanna

In an experiment conducted on in vitro propagation of Asiatic Lilium hybrida cv. Pollyanna. Bulb scales were used as the explants. In vitro culture media for efficient regeneration of Lilium bulb scales were standardized. Pre-treatment with carbendazim (0.2%) + 8-HQC (200 ppm) + GA₃ (100 ppm) for 2 hours followed by surface sterilization with 0.1% mercuric chloride for 7 min. resulted highest healthy cultures (80%). MS medium supplemented with 0.2 mgl⁻¹ NAA and 1.0 mgl⁻¹BAP was the most effective combination for inducing and shoots proliferation. Rooting was best achieved on half-strength MS medium supplemented with 1.0 mgl⁻¹ IBA, where the rooting was 100%.