Department of Horticulture, G.B. Pant University of Agriculture & Technology, Pantnagar, Udham Singh Nagar-263145 (U.P.)
*Present address: Department of Foriculture and Landscaping, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan-173 230.
In vitro propagation of gladiolus cv. American Beauty revealed that cormel tips are excellent explants for culture establishment in MS medium when surface sterilized with 0,1% HgCl2 for 10 min than leaf and inflorescence segments. BAP at 3 mg 1−1 when added to basal medium took 8.4 days for explant establishment, produced more number (4.4) of initial shoots and earliest (14.2 days) to shoot elongation. MS supplemented with BAP 1 mg+ NAA 0.5 mg was found to be optimal for further shoot multiplication. The best rooting was achieved by suplementing basal medium with NAA 0.5 mg. Higher concentration of NAA beyond this inhibited root elongation significantly. A sterilized potting mixture containing FYM + sand + loam (2:1:1, v/v) resulted 60% survival ratio of in vitro rooted plantelets after transplantation.
Gladiolus, in vitro propagation, MS medium, BAP, NAA
