Department of Floriculture and Landscaping, Dr. Y.S. Parmar University of Horticulture and Forestry Nauni, Solan (H.P.) 173 230
*Present address: 563, Sunheri Bagh, Sector-13, Rohm, Delhi-110 085.
Protocol for in vitro rapid clonal multiplication of Dendrobium cv. Sonia was investigated. Maximum establishment of cultures of offest segments occurred on Murashige and Skoog (MS) medium containing 2 µM NAA + 5 µM BAP or 2 µM NAA + 10 µM KIN. MS medium supplemented with 2 µM NAA + 6 µM BAP + 3.4 µM paclobutrazol (PP-333) was optimum for shoot multiplication. Abundant protocom like bodies (Plb's) were formed in MS medium containing 10 µM each of NAA and BAP; these were excellent for further multiplication of shoots. NAA (6 or 8 µM) or IBA (4 or 6 µM) in MS medium was suitable for the rooting of in vitro multiplied shoots.
Dendrobium, paclobutrazol (PP-333), tissue culture, micropropagation
