Detection of Gladiolus Mosaic Virus in Corms, cormels And Different Parts of a Plant Using Dot-Immunobinding Assay (DIBA)

Gladiolus mosaic virus was detected in corms of all the 33 cultivars and cormels of 50 out of 55 cultivars using Dot-immunobinding assay (DIBA). Cutting the corms and incubating for some time was not a pre-requisite for detection in DIBA. The virus was also detected in leaves, inflorescence stalk, sepals, petals, anther, stigma, immature seeds and outer coat of capsule of cultivars Mayur, Melody, Pusa Suhagin and Suchitra. The virus is strongly suspected to be seed-borne.