Precocious Shoot Proliferation and Microcorm Production in Gladiolus Through Tissue Culture

In vitro studies were conducted for rapid clonal multiplication and microcorm production in gladiolus cvs Eurovision and Wine & Roses. Cormels were surface sterilized with mercuric chloride 0.1% for 8–10 minutes. MS medium supplemented with 2 mM NAA, 10–20 mM BAP and 2 mM GA₃ was optimal for the culture establishment of both cultivars. Early sprouting as well faster increase in sprout length occurred with the addition of GA₃ in the establishment medium. Profuse shoot proliferation was obtained on GMM-7(MS+2 mM KIN) in cv. Wine & Roses while it was optimal on GMM-8 (MS + 4 mM KIN) in case of cv. Eurovision. Half strength MS medium (liquid) containing 10 mM IB A, 5 mM KIN and 10 mM PBZ was most satisfactory for profuse microcorm production when kept under dark environment.