Effect of different growth regulators on fresh and dry weight of callus and regeneration in chrysanthemum cultivars Miss Universe and Snow Ball

The effect of different concentrations of 2,4-D, NAA and Kinetin was studied on callus of node, young leaves and intenodal explant of chrysanthemum cvs. Miss Universe and Snow Ball. Among various explants of both cultivars, nodal explants showed the highest fresh weight in two combinations, internode and young leaf in one combination. Dry matter accumulation was more in nodal explant followed by young leaves and interode in both cultivars. Out of different media combinations, MS + 2,4-D (2.0 mg/1) + kinetin (1.0 mg/I) was superior in terms of fresh weight and dry matter accumulation in all explants of both genotypes. Regeneration of shoots took place on MS media fortified with various combinations of BAP and NAA. Rooting of shoots could be achieved by culturing on MS medium supplemented with NAA (1.0 mg/1). The rooted plants were transferred to sterilized sand and after 60 days maintained under high moisture condition. These were then successfully shifted to earthen pots in greenhouse and later on to Botanical Garden of C.C.S.H.A.U., Hisar.