Callus mediated regeneration from petiole explants of Anthurium andreanum cultivar Singapore Hybrid

An efficient regeneration system through callus intermediate from petiole explants is reported for Anthurium andreanum cv. Singapore Hybrid. Cultures were initiated in MS medium with varying concentrations of IBA and Kinetin. Prolific callusing and early regeneration was optimized on IBA (2 me/l) and Kinetin (6 mg/l) treated cultures. Ninety Lpercentpetiokexplants responded forcallusmg within 3llysofcu.turin_g and regen-eration of plantlets was observed after 61 days of culturing. A regeneration frequency of 20.50 shootlets/explant was achieved on MS medium with 2 mg/1 of IBA + 6 mg/1 of Kinetin. Roots developed when regenerated shoots were cultured on MS medium with reduced concentration of cytokinin and sucrose. MS medium supplemented with 1.5 mg/1 of IBA + 1.5 mg/1 of Kinetin was found to be the superior for in vitro rooting followed by 1.5 mg/1 of IBA + 1.0 mg/1 of Kinetin and 1.0 mg/1 of IBA + 1.5 mg/1 of Kinetin. The complete plantlets with 4–6 leaves and 3–5 roots were transferred to sterilized hardening medium consisting of 2:1:1:1 proportion of coffee cherry husk: FYM: soil: sand, respectively. Plants were hardened with a survival rate of 80%.