In vitro regeneration of miniature rose (Rosa chinensis) cultivar Rosamini

An in vitro procedure for large scale multiplication of miniature rose (Rosa chinensis) cv. Rosamini has been developed using nodal segments. An average of 18 shoots/culture were obtained on MS medium containing lmg/1 BA within 23 days of initial culture. Upon subsequent sub-culture, 16 shoots/cluster could be harvested every three weeks. More than 90% shoots were successfully rooted. Rooted plantlets were transferred to plastic pots containing soil and leaf mould (1:1) medium under greenhouse condition before they were finally exposed to external environment Eighty three per cent plants were survived in the nursery. Upon successful field transferred uniform flowering was observed.