Effect of growth regulators on plant regeneration from leaf derived callus in Petunia hybrida

Calli were induced from leaf explant of Petunia hybrida nana compacta on MS medium supplemented with 2,4-D, NAA and IAA. 2, 4-D was most effective in inducing callus. Creamy friable calli induced with 2,4-D at low concentration (1.5 mg/1) were capable of regeneration and shoot formation. A multiplication rate of 16–18 shoots were achieved on MS medium supplemented with 2 mg/1 BA. Regenerated shoots were induced to form roots when cultured on MS medium supplemented with 1 mg/1 NAA. Young plantlets were successfully transferred to community pots for field evaluation.