Influence of Potting Media on Acclimatization and Growth of in Vitro Plantlets of Cymbidhim Hybrids

Studies on the effect of different substrates viz., coco pith, coco pith + leaf mould, coco pith + leaf mould + tree fern, coco pith + leaf mould + tree fern + bricks pieces, coco pith + leaf mould + tree fern + bricks pieces + charcoal, coco pith + sand and leaf mould, on establishment and growth of tissue culture regenerated plants was carried out to find out the suitable media for ex vitro hardening and growth of in vitro raised plantlets of Cymbidhim hybrids. The results showed that the plantlets grown in coco pith resulted in maximum increase in shoot length (15.02%) with broad leaf, whereas the plantlets grown in coco pith along with sand resulted in maximum increase in leaf number (42.31 %) and leaf length (32.24%). Plantlets grown in leaf mould resulted in maximum mortality. Thus, coco pith alone and in combination with sand is best suitable for the acclimatization of in vitro raised seedlings of Cymbidium hybrids Lunavian Atlas, Golden Girl and Cymbidhim lownmum X Show Girl.