Effect of different media and various explants on in vitro callus induction of different cultivars of carnation (Dianthus caryophyllus L.)

Callus induction studies indicated that 100.00 per cent callus induction was observed in all the four cultivars (Cherry Frill, Dona Brecas, Pinto Yellow and Impala) and explants (Shoot tips, Nodal stem segments, Intermodal stem segments and leaf segments) used in vitro. Maximum callus induction from explant shoot tips was observed on MSS₁, MSS₂ and MSS₃ media (Containing MS basal salts (full Strength)+BAP 1.5 mg with 2.0, 2.5 and 3.0 mg 2, 4-D in MSS₁, MSS₂ and MSS, media respectively) in cultivar ‘Cherry Fill', and on MSS₁, MSS₄ (MS basal salts+0.5 mg BAP+0.5 mg 2,4-D) and MSS₅ (MS basal salts+0.5 mg 2, 4-D) media in cultivar ‘Dona Brecas', on MSS₆ (MS basal Salts +1.0 mg 2,4-D) in cultivar ‘Pinto Yellow' and on MSS₃ and MSS₅ in cultivar ‘Impala'. From explant leaf segments and intemodal stem segments maximum callus induction was observed on MSS₅ medium in all the four cultivars. Organogenesis was observed only in cultivar ‘Cherry Frill' on MSL₄ medium (MS basal salts+BAP 1.5 mg +NAA 6.0 mg per litre).