Department of Floriculture and Landscape Gardening, Kittur Rani Channamma College of Horticulture, Arabhvi-591 310
Online published on 1 March, 2012.
An investigation was taken up to standardize a suitable medium-term conservation strategy for gladiolus cv. American Beauty. The influence of media strength, sucrose, alar, mannitol and type of enclosure on in vitro slow growth of gladiolus micro-shoots was studied. Quarter strength MS medium influenced the growth of micro-shoots. Exclusion of sucrose in MS media was effective in delaying the number of days for bud sprouting (23.00days), increasing the storage period (238.60days) and per cent recovery (92.00%). Alar at 40 mg/litre delayed the number of days for bud sprouting (22.80 days) and extended the storage period (158.00 days). Two per cent mannitol effectively delayed the number of days for bud sprouting, reduced shoot length, and increased the storage period. The vessels enclosed by polypropylene cap stored the cultures for 218.20 days with 92.00% recovery.
Gladiolus, in vitro, slow growth, media strength, sucrose, alar, mannitol, enclosure
