Department of Floriculture and Landscape Gardening, Kittur Rani Channamma College of Horticulture, Arabhvi-591 310
Online published on 1 March, 2012.
In vitro multiplication through tissue culture technique is an urgent need tc bypass the dormancy for bulking of new cultivars or disease -free stocks to provide sufficient planting materials for field planting. The one per cent mercuric chloride (HgCl2) for 10 minutes was beneficial as surface sterilizing agent BAP at 2mg/l was suitable for culture establishment which proliferated maximum shoots (3.22), highest per cent bud sprouts (100.0%) and highest shoot length (6.81cm). For rooting, MS medium supplemented with IBA at 0.50mg/l was found to be the most ideal. MS at half strength influenced the root induction.
In vitro, gladiolus, proliferation, media strength
