In vitro propagation and artificial seed production io Arundina bambusifolia

The germination response of Arundina bambusifolia embryos was tested in Nitsch based medium supplemented with various concentrations and combinations of IA A, NAA and complex organic 0.5 mg/1 additives. Results showed that Nitsch media supplemented with 1 mg/1IAA + 0.5 mg/1 NAA + 1 mg/1 BAP + 0.5 mg/1 ME was found to be the best for seed germination (91.67%) and protocorm development (6.33 week). The longest shoot (4.93cm) and root (3.23cm) were observed in IAA (1 mg/1) in conjunction with NAA (05 mg/1). Artificial seeds stored at 4°C by embedding with liquid parafin, when recultured at one month interval showed shoot and root emergence frequencies from 70–90%, which was observed upto 120 day of storage.