Depatment of Floriculture and Landscaping, Horticultural College anil Research Institute, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, 641003, e-mail: flowers@tnau.ac.in
A study was conducted to establish a protocol for large scale clonal propagation of chrysanthemum (Dendranthema grandiflom Tzvelev) cv. Co.l. The frequncy of multiple shoot regeneration response was 95% and 80% for nodal segments and shoot tips, when cultured on the media containing MS+ 20 mg/l BAP +1.0 mg/l IB A. In vitro raised plantlets were successfully transfrred to potting media and got established in the main field.
BAP, chrysanthemum, IBA, micro propagaion, in vitro
