1Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi-110012
2Department of Biotechnology, A.P.S. University, Rewa-486 001, Madhya Pradesh, e-mail: singhsk@iari.res.in
3Division of Floriculture and Landscaping, Indian Agricultural Research Institute, New Delhi-110012
4Division Fruits and Horticultural Technology, Indian Agricultural Research Institute, New Delhi-110012
An efficient protocol for large scale multiplication of chrysanthemum (Dendrathetna grandiflora Tzevelev.) cv. Yellow Bungalow was standardized. For culture initiation and surface sterilization, pre-treatment (Carbendazim,- 0.2% + 8-hydroxy quinnoline citrate (HQC) -200 ppm) for 2 h followed by 3 min. agitation in 0.1% HgCl2 gave good success for both shoot tip and nodal segment explants. The maximum shoot proliferation (4.43 shootlets/explant) was achieved on MS medium supplemented with BAP 3.0 mg/1 and NAA 0.01 mg/I after 15-day interval on shoot tip explants. The mean shoot multiplication rate for ten sub-cultures was achieved maximum (over 12.0 shoots per explant/subculture) on the above medium supplemented with GA, (0J mg/1). Rooting of micro-shoots was achieved best on half-strength MS medium supplemented with 03 mg/1 NAA and 60 g/l sucrose. These plantlets were successfully transferred into pots filled with sterile agro-peat supplemented with half-strength MS liquid for acclimatization in glass jar giving rise to over 95 % survival.
Chrysanthemum, micropropagation, nodal segment, shoot tip
