1Division of Fruits and Horticultural Technology,
2Division of Floriculture and Landscaping, Indian Agicultural Research Institute, New Delhi-110012. e-mail: singhsk@iari.res.in
An efficient protocol for micro-propagation of miniature rose (Rosa chinensis Jacq. var. minima) cvs. Candy Sunblaze and Baby Katie has been established using nodal segments. Of the different pre-treatments tried, the maximum response (82.3%) was obtained with 1000 mgf/l Ridomil + 200 mgf/l 8-HQC for 2 h agitation. Cultures were initiated on Murashige and Skoog medium supplemented with different plant growth regulators. MS basal medium fortified with 4.0 mg/l BAP + 2.0 mg/1 kinetin and 0.1 mg/l NAA, gave the highest shoot multiplication rate (6.9 shoots/explant). In vitro rooting percentage was enhanced by use of half-strength MS basal medium fortified with 1.0 mg/l IBA + 1.0 mg/1 NAA. The regenerated plantlets were best acclimatized in glass jar filled with peat: soilrite (1:1) with PC cap. After acclimatization, the plants were successfully transferred to the glasshouse.
Micro-propagation, miniature rose, protocol
