Rapid and highly efficient In vitro regeneration protocol for cowpea (Vigna unguiculata (L.) Walp.)

The cotyledons node explants of Vigna unguiculata variety GC-4 were cultured on Murashige & Skoog (MS) medium supplemented with 0-5mg/liter BAP. All treatments produced shoot. Most rapid growth and number of shoots were achieved through 1mg BAP/liter. Rhizogenesis was recorded in MS medium with IBA (0.5–5 mg/liter). The highest rooting (89.56%) was observed on medium supplemented with 5 mg/liter of IBA under continuous light conditions. Higher plantlet regeneration was obtained using cotyledonary node explants in Cow pea than any other explants.